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growth/differentiation factor 5 (gdf-5)  (PeproTech)


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    PeproTech growth/differentiation factor 5 (gdf-5)
    Growth/Differentiation Factor 5 (Gdf 5), supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/growth+and+differentiation+factor+5+gdf-5/gdf5+growth+factor/pm38734200-72-41-38
    Average 90 stars, based on 1 article reviews
    growth/differentiation factor 5 (gdf-5) - by Bioz Stars, 2026-11
    90/100 stars

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    Concentration Assay:

    Article Title: In vitro and in vivo evaluation of periosteum-derived cells and iPSC-derived chondrocytes encapsulated in GelMA for osteochondral tissue engineering
    Article Snippet: Next, the formation of cartilage-like nodules was stimulated by culturing the cells in a chondrogenic medium consisting of DMEM GlutaMAXTM with 1 mM pyruvate supplemented with 1% FBS, 1% L-glutamine (Thermo Fisher, United States), 1% MEM Non-Essential Amino Acids Solution (100×) (Gibco, United States), 0.05 ng mL -1 ascorbic acid L-variant (Sigma, DE), 50 U mL -1 penicillin–streptomycin (Gibco, United States), 82.5 μM β-mercaptoethanol (Gibco, United States), and 1% insulin–transferrin–selenium–ethanolamine (ITS-X) (100×) (Gibco, United States). .. Growth factors were added at a concentration of 10 ng mL -1 : transforming growth factor β1 (TGF-β1; PeproTech, United Kingdom), growth and differentiation factor 5 (GDF-5; PeproTech, United Kingdom), bone morphogenetic protein 2 (BMP-2; PeproTech, United Kingdom), and hbFGF (PeproTech, United Kingdom) for 2 weeks. ..



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    ( A,B ) Neurite length of neurons in E14 rat VM cultures transfected with control siRNA, Smad4 siRNA or Smad1 siRNA or Smad5 siRNA and a GFP-expressing plasmid before being cultured with or without 10 ng/ml <t>GDF5</t> for 72 h (*** P <0.001 compared with control; ANOVA with post-hoc Tukey’s test; 40 cells for each group per experiment; number of repetitions ( n )=3 experiments). All data are presented as mean ± S.E.M. ( C ) Representative photomicrographs of GFP+ neurons in cultures of E14 rat VM. Scale bar as indicated.
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    ( A,B ) Neurite length of neurons in E14 rat VM cultures transfected with control siRNA, Smad4 siRNA or Smad1 siRNA or Smad5 siRNA and a GFP-expressing plasmid before being cultured with or without 10 ng/ml GDF5 for 72 h (*** P <0.001 compared with control; ANOVA with post-hoc Tukey’s test; 40 cells for each group per experiment; number of repetitions ( n )=3 experiments). All data are presented as mean ± S.E.M. ( C ) Representative photomicrographs of GFP+ neurons in cultures of E14 rat VM. Scale bar as indicated.

    Journal: Neuronal Signaling

    Article Title: Inhibition of miR-181a promotes midbrain neuronal growth through a Smad1/5-dependent mechanism: implications for Parkinson’s disease

    doi: 10.1042/NS20170181

    Figure Lengend Snippet: ( A,B ) Neurite length of neurons in E14 rat VM cultures transfected with control siRNA, Smad4 siRNA or Smad1 siRNA or Smad5 siRNA and a GFP-expressing plasmid before being cultured with or without 10 ng/ml GDF5 for 72 h (*** P <0.001 compared with control; ANOVA with post-hoc Tukey’s test; 40 cells for each group per experiment; number of repetitions ( n )=3 experiments). All data are presented as mean ± S.E.M. ( C ) Representative photomicrographs of GFP+ neurons in cultures of E14 rat VM. Scale bar as indicated.

    Article Snippet: Where indicated, cells were treated daily with 10 or 200 ng/ml of growth differentiation factor (GDF) 5 (GDF5) (Biopharm GmbH), which are maximal saturating concentrations in primary and SH-SY5Y cell cultures respectively [ , ].

    Techniques: Transfection, Control, Expressing, Plasmid Preparation, Cell Culture

    ( A ) Densitometric analysis of p-Smad1/5 in SH-SY5Y cells treated with 200 ng/ml of GDF5 for 0 (control), 5, 30 and 60 min. ( B ) Neurite length of GDF5-treated (200 ng/ml daily for 72 h) SH-SY5Y cells at 72 h. ( C–F ) p-Smad1/5 levels as determined by ( C, D ) Western blots or ( E, F ) immunocytochemistry in SH-SY5Y cells 24 h after transfection with control miRNA or miR-181a inhibitor. Total Smad1/5 was used as a loading control. ( G ) Neurite length, ( H ) neurite branching and ( I ) representative photomicrographs of miR-181a inhibitor or control miRNA transfected SH-SY5Y cells at 72 h (* P <0.05, ** P <0.01, *** P <0.001 compared with control; t test; 40 cells for each group per experiment; n =3 experiments). All data are presented as mean ± S.E.M. Scale bar as indicated.

    Journal: Neuronal Signaling

    Article Title: Inhibition of miR-181a promotes midbrain neuronal growth through a Smad1/5-dependent mechanism: implications for Parkinson’s disease

    doi: 10.1042/NS20170181

    Figure Lengend Snippet: ( A ) Densitometric analysis of p-Smad1/5 in SH-SY5Y cells treated with 200 ng/ml of GDF5 for 0 (control), 5, 30 and 60 min. ( B ) Neurite length of GDF5-treated (200 ng/ml daily for 72 h) SH-SY5Y cells at 72 h. ( C–F ) p-Smad1/5 levels as determined by ( C, D ) Western blots or ( E, F ) immunocytochemistry in SH-SY5Y cells 24 h after transfection with control miRNA or miR-181a inhibitor. Total Smad1/5 was used as a loading control. ( G ) Neurite length, ( H ) neurite branching and ( I ) representative photomicrographs of miR-181a inhibitor or control miRNA transfected SH-SY5Y cells at 72 h (* P <0.05, ** P <0.01, *** P <0.001 compared with control; t test; 40 cells for each group per experiment; n =3 experiments). All data are presented as mean ± S.E.M. Scale bar as indicated.

    Article Snippet: Where indicated, cells were treated daily with 10 or 200 ng/ml of growth differentiation factor (GDF) 5 (GDF5) (Biopharm GmbH), which are maximal saturating concentrations in primary and SH-SY5Y cell cultures respectively [ , ].

    Techniques: Control, Western Blot, Immunocytochemistry, Transfection